Tehran University of Medical Sciences International Journal of Occupational Hygiene 2008-5109 3 1 2011 06 15 12 17 Abdulrahman Bahrami Department of Occupational Health, Research Centre for Health Sciences, School of Public Health, Hamedan University of Medical Sciences, Hamedan, Iran Hossien Mahjub 2Department of Biostatics and Epidemiology, Research Centre for Health Sciences, School of Public Health, Hamedan University of Medical Sciences, Hamedan, Iran Marzieh Sadeghian Department of Occupational Health, Research Centre for Health Sciences, School of Public Health, Hamedan University of Medical Sciences, Hamedan, Iran Farideh Golbabaei Department of Occupational Health, Faculty of Health, Tehran University of Medical Sciences, Tehran, Iran 2015 10 04 A new method for analysis of benzene, toluene, and xylene (BTX) using High Performance Liquid Chromatography-UV detection (HPLC-UV) is described and compared to the gas chromatography (GC) method. A charcoal adsorption tube connected to a small pump was used to obtain samples from an atmosphere chamber standard. Samples were extracted with methanol and analyzed by HPLC-UV. Chromatography was isocratic in a mobile phase consisting of water-methanol (30-70). The flow rate was set at 1 ml/min. The analyses were completely separated and were quantified using both methods. The results demonstrated no statistically significant differences between BTX concentrations between the two analytical methods with a correlation coefficient of 0.98-0.99. The GC method provided higher sensitivity than HPLC, but the HPLC determination of BTX were applicable to real samples because its sensivity was lower than the thershold limit recommended by the American Conference of Governmental Industrial Hygienist (ACGIH) for an 8-hour workday. https://ijoh.tums.ac.ir/index.php/ijoh/article/view/27 https://ijoh.tums.ac.ir/index.php/ijoh/article/download/27/27