Tehran University of Medical Sciences International Journal of Occupational Hygiene 2008-5109 17 1 2025 03 03 Effect of Supplementation with Vitamin E and Omega-3 Fatty Acids on Oxidative Stress Parameters of Workers Exposed to High-Level Noise 40 54 Hamzeh Mohammadi Islamic Azad University Farideh Golbabaei Tehran University of Medical Sciences Somayeh Dehghan Shahid Beheshti University of Medical Sciences Nariman Moradi Kurdistan University of Medical Sciences Maryam Chamari Tehran University of Medical Sciences 2024 11 14 2024 12 30 Background: The present study aimed to assess the effects of vitamin E and Omega-3 fatty acid supplementation on oxidative stress parameters among workers exposed to high noise levels in an automobile parts manufacturing plant. Additionally, the effects of noise exposure on superoxide dismutase (SOD), total antioxidant capacity (TAC), and malondialdehyde (MDA) were investigated. Method: Participants were divided into four groups (vitamin E [100 mg], Omega-3 fatty acids [180 mg EPA and 120 mg DHA], vitamin E + Omega-3, and Placebo) using a double-blind block randomization method. Oxidative stress parameters were analyzed before and after three months of supplementation using the enzyme-linked immunosorbent assay method. Workers’ noise exposure levels were measured according to ISO 9612. Results: Before the intervention, mean MDA, SOD, and TAC levels were 27.52 (7.46) nmol/ml, 58.84 (10.44) U/ml, and 2.57 (0.67) mM, respectively. After the intervention, mean MDA, SOD, and TAC levels were 24.57 (7.58) nmol/ml, 63.46 (11.02) U/ml, and 2.70 (0.84) mM, respectively. Omega-3 fatty acid supplementation significantly decreased MDA levels. Moreover, vitamin E combined with Omega-3 fatty acids significantly increased SOD activity. Noise exposure decreased TAC and SOD levels and increased MDA levels, but the changes were only statistically significant for TAC. Conclusion: The simultaneous use of vitamin E and Omega-3 fatty acids for three months positively affected the antioxidant performance of workers exposed to noise. https://ijoh.tums.ac.ir/index.php/ijoh/article/view/657 https://ijoh.tums.ac.ir/index.php/ijoh/article/download/657/819