Tehran University of Medical Sciences International Journal of Occupational Hygiene 2008-5109 5 4 2015 10 11 177 183 EN NEMATULLAH KURD Department of Occupational Health Engineering, Hamedan University of Medical Sciences, Hamadan, Iran. ABDULRAHMAN BAHRAMI Research Centre for Health Sciences, Faculty of Health, Hamedan University of Medical Sciences, Hamadan, Iran. FARSHID GHORBANI-SHAHNA Research Centre for Health Sciences, Faculty of Health, Hamedan University of Medical Sciences, Hamadan, Iran. MAHMOUD HEIDARI Department of Occupational Health, Faculty of Health, Guilan University of Medical Sciences, Rasht, Iran. 2015 10 11 2015 10 11 Acetonitrile used as a solvent in manufactures and affects to central nervous system from inhalation exposure. The aim of this study was to develop a micro-solid phase extraction method for  the determination of acetonitrile in the air matrix. The sampling was performed with a small diameter fused silica fiber coated with a thin film of stationary phase and was subsequently desorbed and analyzed by gas chromatograph equipped with a flame ionization detector (GC/FID). The effects of laboratory and sampling parameters were investigated and applied to the determination of acetonitrile in air matrix. The Carboxen/PDMS  as the coating fiber showed better analytical performances compared to the PDMS fiber. Analysis of the data by ANOVA test at a 0.05 level of accuracy showed that the peak area of the sampler was significantly affected by temperature and humidity so that the optimum temperature was 20°C and the optimum humidity was 35%. Besides, the limit of detection (LOD) and limit of quantification (LOQ) for acetonitrile in the GC system were 0.05 and 0.15 μg/ml, respectively. The solid phase  microextraction (SPME) has been shown a suitable technique for sampling and analysis of acetonitrile in air. There was a good correlation between the SPME and national institute occupational safety and health (NIOSH) 1010 method under the optimum conditions. https://ijoh.tums.ac.ir/index.php/ijoh/article/view/86 https://ijoh.tums.ac.ir/index.php/ijoh/article/download/86/86