Effect of Supplementation with Vitamin E and Omega-3 Fatty Acids on Oxidative Stress Parameters of Workers Exposed to High-Level Noise
-
Hamzeh Mohammadi
,
Farideh Golbabaei
,
Somayeh Farhang Farhang Dehghan
,
Nariman Moradi
,
Maryam Chamari
Abstract
Background: The present study aimed to assess the effects of vitamin E and Omega-3 fatty acid supplementation on oxidative stress parameters among workers exposed to high noise levels in an automobile parts manufacturing plant. Additionally, the effects of noise exposure on superoxide dismutase (SOD), total antioxidant capacity (TAC), and malondialdehyde (MDA) were investigated.
Method: Participants were divided into four groups (vitamin E [100 mg], Omega-3 fatty acids [180 mg EPA and 120 mg DHA], vitamin E + Omega-3, and Placebo) using a double-blind block randomization method. Oxidative stress parameters were analyzed before and after three months of supplementation using the enzyme-linked immunosorbent assay method. Workers’ noise exposure levels were measured according to ISO 9612.
Results: Before the intervention, mean MDA, SOD, and TAC levels were 27.52 (7.46) nmol/ml, 58.84 (10.44) U/ml, and 2.57 (0.67) mM, respectively. After the intervention, mean MDA, SOD, and TAC levels were 24.57 (7.58) nmol/ml, 63.46 (11.02) U/ml, and 2.70 (0.84) mM, respectively. Omega-3 fatty acid supplementation significantly decreased MDA levels. Moreover, vitamin E combined with Omega-3 fatty acids significantly increased SOD activity. Noise exposure decreased TAC and SOD levels and increased MDA levels, but the changes were only statistically significant for TAC.
Conclusion: The simultaneous use of vitamin E and Omega-3 fatty acids for three months positively affected the antioxidant performance of workers exposed to noise.
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15. Hahad O, et al. Environmental noise‐induced effects on stress hormones, oxidative stress, and vascular dysfunction: Key factors in the relationship between cerebrocardiovascular and psychological disorders. 2019;2019(1):4623109.
16. Aslani BA, Ghobadi S. Studies on oxidants and antioxidants with a brief glance at their relevance to the immune system. Life Sci. 2016;146:163-173.
17. Shahi M, Nejati V, Najafi G. Effect of Omega-3 on Ovarian Tissue and Blastocyst Formation on Diabetic Rats In Vitro. J Mazandaran Univ Med Sci. 2017;26(145):176-185.
18. Lands B. Omega-3 PUFAs lower the propensity for arachidonic acid cascade overreactions. Biomed Res Int. 2015;2015.
19. Anghel L, et al. The Therapeutic Relevance of Vitamin E. Rev Chim. 2019;70(10):3711-3713.
20. Surai PF, Kochish II. Nutritional modulation of the antioxidant capacities in poultry: the case of selenium. Poult Sci. 2019;98(10):4231-4239.
21. Camkurt MA, et al. Evaluation of malondialdehyde, superoxide dismutase and catalase activity in fetal cord blood of depressed mothers. Clin Psychopharmacol Neurosci. 2017;15(1):35.
22. Mohammadi H, et al. Assessment of sexual hormones in foundry workers exposed to heat stress and electromagnetic fields. Reprod Toxicol. 2021;101:115-123.
23. Mohammadi H, et al. Relationship between occupational exposure to whole-body vibration and noise with sex hormone levels: An empirical assessment in an automobile parts manufacturing plant. Toxicol Ind Health. 2021.
24. Keskes-Ammar L, et al. Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men. Arch Androl. 2003;49(2):83-94.
25. Nassiri P, et al. The assessment of the environmental noise and personal exposure in a petrochemical plant. Iran Occup Health. 2013;10(1):23-32.
26. ACGIH. TLVs and BEIs, threshold limit values for chemical substances and physical agents and biological exposure indices. Cincinnati, OH: Signature Publications; 2021.
27. Mohammadi H, et al. Evaluation of potential biomarkers of exposure to crystalline silica: A Case Study in an Insulator Manufacturer. Toxicol Ind Health. 2018;34(7):491-498.
28. Joshaghani H, Shafiee A. Correlation between serum and erythrocyte superoxide dismutase with serum homocysteine in myocardial infarction. Q Horiz Med Sci. 2007;13(1):5-9.
29. Prohan M, et al. Total antioxidant capacity of diet and serum, dietary antioxidant vitamins intake, and serum hs-CRP levels in relation to depression scales in university male students. Redox Rep. 2014;19(3):133-139.
30. Elsayed NM, Gorbunov NV. Interplay between high energy impulse noise (blast) and antioxidants in the lung. Toxicology. 2003;189(1-2):63-74.
31. Haghighat M, et al. Effects of concomitant exposure to styrene and intense noise on rats’ whole lung tissues. Biochemical and histopathological studies. Drug Chem Toxicol. 2019:1-7.
32. Hosseinabadi MB, et al. The effects of industrial noise exposure on lipid peroxidation and antioxidant enzymes among workers. Int Arch Occup Environ Health. 2019;92(7):1041-1046.
33. Yildirim I, et al. The effects of noise on hearing and oxidative stress in textile workers. Ind Health. 2007;45(6):743-749.
34. Demirel R, et al. Noise induces oxidative stress in rat. Eur J Gen Med. 2009;6(1):20-24.
35. Pournourmohammadi S, et al. Study on the oxidative stress status among cement plant workers. Hum Exp Toxicol. 2008;27(6):463-469.
36. Ranjbar A, Barkhordari A, Dehghani A. The study of oxidative stress biomarkers in ceramic workers compared to control group. Occup Med Q J. 2016;7(4):67-81.
37. Hassan Eftekhari M, et al. Effect of conjugated linoleic acid and omega-3 fatty acid supplementation on inflammatory and oxidative stress markers in atherosclerotic patients. ARYA Atherosclerosis. 2013;9(6):311-318.
38. Tayebi KH, et al. Effect of omega-3 fatty acid on oxidative stress in patients on hemodialysis. Iran J Kidney Dis. 2010;4(4):322-326.
39. Fazelian S, et al. Effect of omega-3 fatty acids supplementation on cardio-metabolic and oxidative stress parameters in patients with chronic kidney disease: a systematic review and meta-analysis. BMC Nephrol. 2021;22(1):160.
40. Jamilian M, Ravanbakhsh N. Effects of Vitamin E plus Omega-3 Supplementation on Inflammatory Factors, Oxidative Stress Biomarkers and Pregnancy Consequences in Women with Gestational Diabetes. J Arak Univ Med Sci. 2018;21(5):32-41.
41. Rahmani E, et al. The effects of omega-3 fatty acids and vitamin E co-supplementation on gene expression of lipoprotein (a) and oxidized low-density lipoprotein, lipid profiles and biomarkers of oxidative stress in patients with polycystic ovary syndrome. Mol Cell Endocrinol. 2017;439:247-255.
42. Liu Q, et al. Effects of dietary n-6: n-3 fatty acid ratio and vitamin E on semen quality, fatty acid composition and antioxidant status in boars. Anim Reprod Sci. 2015;162:11-19.
43. Ko EY, Sabanegh ES Jr, Agarwal A. Male infertility testing: reactive oxygen species and antioxidant capacity. Fertil Steril. 2014;102(6):1518-1527.
44. Riediger ND, et al. A systemic review of the roles of n-3 fatty acids in health and disease. J Am Diet Assoc. 2009;109(4):668-679.
45. Mazlomi A, et al. The influence of occupational heat exposure on cognitive performance and blood level of stress hormones: a field study report. Int J Occup Saf Ergon. 2017;23(3):431-439.
2. Daiber A, Münzel T. Special Issue: Impact of environmental pollution and stress on redox signaling and oxidative stress pathways. Redox Biol. 2020;37.
3. Jakubczyk K, et al. Reactive oxygen species—Sources, functions, oxidative damage. Pol Merkur Lekarski. 2020;48(284):124-127.
4. Queiroz EKRd, Waissmann W. Occupational exposure and effects on the male reproductive system. Cad Saude Publica. 2006;22(3):485-493.
5. Ighodaro O, Akinloye O. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria J Med. 2018;54(4):287-293.
6. Poljsak B, Šuput D, Milisav I. Achieving the balance between ROS and antioxidants: when to use the synthetic antioxidants. Oxid Med Cell Longev. 2013;2013.
7. Poprac P, et al. Targeting free radicals in oxidative stress-related human diseases. Trends Pharmacol Sci. 2017;38(7):592-607.
8. Basner M, et al. Auditory and non-auditory effects of noise on health. Lancet. 2014;383(9925):1325-1332.
9. Munne-Bosch S, Pinto-Marijuan M. Free radicals, oxidative stress and antioxidants. Encycl Appl Plant Sci. 2016;2(H2O2):16.
10. Hahad O, et al. Environmental noise‐induced effects on stress hormones, oxidative stress, and vascular dysfunction: Key factors in the relationship between cerebrocardiovascular and psychological disorders. Oxid Med Cell Longev. 2019;2019(1):4623109.
11. Münzel T, et al. The adverse effects of environmental noise exposure on oxidative stress and cardiovascular risk. 2018;28(9):873-908.
12. Münzel T, et al. The adverse effects of environmental noise exposure on oxidative stress and cardiovascular risk. Antioxid Redox Signal. 2018;28(9):873-908.
13. Moradpour Z, et al. Oxidative Stress Assessment of Noise Exposure in the Workers Using Hearing Protection in a Pressing Industry. 2024;25(6):1929.
14. Arregi A, et al. Road traffic noise exposure and its impact on health: evidence from animal and human studies—chronic stress, inflammation, and oxidative stress as key components of the complex downstream pathway underlying noise-induced non-auditory health effects. 2024;31(34):46820-46839.
15. Hahad O, et al. Environmental noise‐induced effects on stress hormones, oxidative stress, and vascular dysfunction: Key factors in the relationship between cerebrocardiovascular and psychological disorders. 2019;2019(1):4623109.
16. Aslani BA, Ghobadi S. Studies on oxidants and antioxidants with a brief glance at their relevance to the immune system. Life Sci. 2016;146:163-173.
17. Shahi M, Nejati V, Najafi G. Effect of Omega-3 on Ovarian Tissue and Blastocyst Formation on Diabetic Rats In Vitro. J Mazandaran Univ Med Sci. 2017;26(145):176-185.
18. Lands B. Omega-3 PUFAs lower the propensity for arachidonic acid cascade overreactions. Biomed Res Int. 2015;2015.
19. Anghel L, et al. The Therapeutic Relevance of Vitamin E. Rev Chim. 2019;70(10):3711-3713.
20. Surai PF, Kochish II. Nutritional modulation of the antioxidant capacities in poultry: the case of selenium. Poult Sci. 2019;98(10):4231-4239.
21. Camkurt MA, et al. Evaluation of malondialdehyde, superoxide dismutase and catalase activity in fetal cord blood of depressed mothers. Clin Psychopharmacol Neurosci. 2017;15(1):35.
22. Mohammadi H, et al. Assessment of sexual hormones in foundry workers exposed to heat stress and electromagnetic fields. Reprod Toxicol. 2021;101:115-123.
23. Mohammadi H, et al. Relationship between occupational exposure to whole-body vibration and noise with sex hormone levels: An empirical assessment in an automobile parts manufacturing plant. Toxicol Ind Health. 2021.
24. Keskes-Ammar L, et al. Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men. Arch Androl. 2003;49(2):83-94.
25. Nassiri P, et al. The assessment of the environmental noise and personal exposure in a petrochemical plant. Iran Occup Health. 2013;10(1):23-32.
26. ACGIH. TLVs and BEIs, threshold limit values for chemical substances and physical agents and biological exposure indices. Cincinnati, OH: Signature Publications; 2021.
27. Mohammadi H, et al. Evaluation of potential biomarkers of exposure to crystalline silica: A Case Study in an Insulator Manufacturer. Toxicol Ind Health. 2018;34(7):491-498.
28. Joshaghani H, Shafiee A. Correlation between serum and erythrocyte superoxide dismutase with serum homocysteine in myocardial infarction. Q Horiz Med Sci. 2007;13(1):5-9.
29. Prohan M, et al. Total antioxidant capacity of diet and serum, dietary antioxidant vitamins intake, and serum hs-CRP levels in relation to depression scales in university male students. Redox Rep. 2014;19(3):133-139.
30. Elsayed NM, Gorbunov NV. Interplay between high energy impulse noise (blast) and antioxidants in the lung. Toxicology. 2003;189(1-2):63-74.
31. Haghighat M, et al. Effects of concomitant exposure to styrene and intense noise on rats’ whole lung tissues. Biochemical and histopathological studies. Drug Chem Toxicol. 2019:1-7.
32. Hosseinabadi MB, et al. The effects of industrial noise exposure on lipid peroxidation and antioxidant enzymes among workers. Int Arch Occup Environ Health. 2019;92(7):1041-1046.
33. Yildirim I, et al. The effects of noise on hearing and oxidative stress in textile workers. Ind Health. 2007;45(6):743-749.
34. Demirel R, et al. Noise induces oxidative stress in rat. Eur J Gen Med. 2009;6(1):20-24.
35. Pournourmohammadi S, et al. Study on the oxidative stress status among cement plant workers. Hum Exp Toxicol. 2008;27(6):463-469.
36. Ranjbar A, Barkhordari A, Dehghani A. The study of oxidative stress biomarkers in ceramic workers compared to control group. Occup Med Q J. 2016;7(4):67-81.
37. Hassan Eftekhari M, et al. Effect of conjugated linoleic acid and omega-3 fatty acid supplementation on inflammatory and oxidative stress markers in atherosclerotic patients. ARYA Atherosclerosis. 2013;9(6):311-318.
38. Tayebi KH, et al. Effect of omega-3 fatty acid on oxidative stress in patients on hemodialysis. Iran J Kidney Dis. 2010;4(4):322-326.
39. Fazelian S, et al. Effect of omega-3 fatty acids supplementation on cardio-metabolic and oxidative stress parameters in patients with chronic kidney disease: a systematic review and meta-analysis. BMC Nephrol. 2021;22(1):160.
40. Jamilian M, Ravanbakhsh N. Effects of Vitamin E plus Omega-3 Supplementation on Inflammatory Factors, Oxidative Stress Biomarkers and Pregnancy Consequences in Women with Gestational Diabetes. J Arak Univ Med Sci. 2018;21(5):32-41.
41. Rahmani E, et al. The effects of omega-3 fatty acids and vitamin E co-supplementation on gene expression of lipoprotein (a) and oxidized low-density lipoprotein, lipid profiles and biomarkers of oxidative stress in patients with polycystic ovary syndrome. Mol Cell Endocrinol. 2017;439:247-255.
42. Liu Q, et al. Effects of dietary n-6: n-3 fatty acid ratio and vitamin E on semen quality, fatty acid composition and antioxidant status in boars. Anim Reprod Sci. 2015;162:11-19.
43. Ko EY, Sabanegh ES Jr, Agarwal A. Male infertility testing: reactive oxygen species and antioxidant capacity. Fertil Steril. 2014;102(6):1518-1527.
44. Riediger ND, et al. A systemic review of the roles of n-3 fatty acids in health and disease. J Am Diet Assoc. 2009;109(4):668-679.
45. Mazlomi A, et al. The influence of occupational heat exposure on cognitive performance and blood level of stress hormones: a field study report. Int J Occup Saf Ergon. 2017;23(3):431-439.
| Files | ||
| Issue | Vol 17 No 1 (2025) | |
| Section | Original Article(s) | |
| Published | 2025-03-03 | |
| Keywords | ||
| Occupational Exposure Noise Oxidative Stress Vitamin E Omega-3 | ||
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How to Cite
1.
Mohammadi H, Golbabaei F, Dehghan SF, Moradi N, Chamari M. Effect of Supplementation with Vitamin E and Omega-3 Fatty Acids on Oxidative Stress Parameters of Workers Exposed to High-Level Noise. Int J Occup Hyg. 2025;17(1):40-54.
